ohn Walker, founder The "Johnnie Walker Bond" in Kilmarnock operated until the opening of a new plant on Hill Street in Kilmarnock

John Walker was born on 25 July 1805. His farmer father died in 1819, and the family sold the farm. Their trustees invested the proceeds, £417, in an Italian warehouse, grocery, and wine and spirits shop on the High Street in Kilmarnock, Ayrshire, Scotland. Walker managed the grocery, wine, and spirits segment as a teenager in 1820. The Excise Act 1823 relaxed strict laws on distillation of whisky and reduced, by a considerable amount, the extremely heavy taxes on the distillation and sale of whisky. By 1825, Walker, a teetotaller, was selling spirits, including rum, brandy, gin, and whisky.

In short order, he switched to dealing mainly in whisky. Since blending of grain whiskies with malt whiskies was still banned, he sold both blended malt whiskies and grain whiskies. They were sold as made-to-order whiskies, blended to meet specific customer requirements, because he did not have any brand of his own. He began using his name on labels years later, selling a blended malt as Walker's Kilmarnock Whisky. John Walker died in 1857.

Death of Walker and takeover by son Alexander Walker, son of John Walker, inherited the business following his father's death.

The brand became popular, but after Walker's death it was his son Alexander 'Alec' Walker and grandson Alexander Walker II who were largely responsible for establishing the whisky as a favoured brand. The Spirits Act 1860 legalised the blending of grain whiskies with malt whiskies and ushered in the modern era of blended Scotch whisky. Blended Scotch whisky, lighter and sweeter in character, was more accessible, and much more marketable to a wider audience. Andrew Usher of Edinburgh, was the first to produce a blended whisky, but the Walkers followed in due course.

Alexander Walker had introduced the brand's signature square bottle in 1860. This meant more bottles fitting the same space and fewer broken bottles. The other identifying characteristic of the Johnnie Walker bottle was – and still is – the label, which, since that year, is applied at an angle of 24 degrees upwards left to right and allows text to be made larger and more visible. This also allowed consumers to identify it at a distance. One major factor in his favour was the arrival of a railway in Kilmarnock, carrying goods to merchant ships travelling the world. Thanks to Alec's business acumen, sales of Walker's Kilmarnock reached 100,000 gallons (450,000 litres) per year by 1862.

In 1865, Alec created Johnnie Walker's first commercial blend and called it Old Highland Whisky, before registering it as such in 1867.

Under John Walker, whisky sales represented eight percent of the firm's income; by the time Alexander was ready to pass on the company to his own sons, that figure had increased to between 90 and 95 percent.